《植物生理学报》 2018, 54(5): 736-744

糖诱导拟南芥侧根发育的QTL分析

贾涛^1,2,#, 彭瑜^2,#, 孙爱清², 秦萍^1,2, 杜如月³, 滕胜^2,*

¹上海大学生命科学学院, 上海200444; ²中国科学院上海植物生理生态研究所分子植物科学卓越创新中心光合与环境实验室, 上海200032; ³洛阳师范学院生命科学学院, 河南洛阳471934

通信作者：滕胜；E-mail: steng@sibs.ac.cn

摘　要：

根系是植物吸收土壤中水分和矿质营养的重要器官, 影响植物的整体生长发育。侧根是植物根系构型的重要因素。植物光合作用产物——糖在侧根原基(LRP)的起始、形成以及伸长阶段发挥了关键作用。本研究发现拟南芥(Arabidopsis thaliana)生态型Bayreuth (Bay-0)和Shahdara (Sha)在侧根发育对葡萄糖的响应中存在显著差异, 利用Bay-0×Sha重组自交系(RIL)群体及分子标记遗传连锁图谱, 在3号染色体短臂和5号染色体长臂定位到两个葡萄糖诱导侧根发育的数量性状位点(QTL), 并在异源近亲代(HIF)中得到验证, 为后续精细定位响应糖信号调控拟南芥侧根发育的主效基因奠定了基础。

关键词：侧根; 糖信号; 数量性状位点; 重组自交系; 异源近亲代

收稿：2018-03-26   修定：2018-05-02

资助：国家自然科学基金(31570269、31570279和31370284)。

QTL mapping of sugar triggered lateral root development in Arabidopsis thaliana

JIA Tao^1,2,#, PENG Yu^2,#, SUN Ai-Qing², QIN Ping^1,2, DU Ru-Yue³, TENG Sheng^2,*

¹School of Life Sciences, Shanghai University, Shanghai 200444, China; ²Laboratory of Photosynthesis and Environmental Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China; ³School of Life Sciences, Luoyang Normal University, Luoyang, Henan 471934, China

Corresponding author: TENG Sheng; E-mail: steng@sibs.ac.cn

Abstract:

Plant roots, which act as an important role in absorbing water and mineral nutrients from soil, affect the development of the whole plant. Lateral roots are essential components of the root architecture. It is well known that sugar which is produced via photosynthesis plays an important role in the initiation, formation and development of lateral root. In this study, we found that there was significant natural variation between two Arabidopsis thaliana ecotypes Bayreuth (Bay-0) and Shahdara (Sha) in lateral root development responsing to glucose treatment. By using the population of recombinant inbred line (RIL) of Bay-0 and Sha combined with molecular linkage map, we find two quantitative trait loci (QTLs) located on the short arm of chromosome 3 and the long arm of chromosome 5, respectively. These two QTLs were verified with heterogeneous inbred family (HIF), which laid the foundation for the follow-up fine mapping of major gene of A. thaliana lateral root development triggered by sugar signaling.

Key words: lateral root; sugar signaling; quantitative trait locus (QTL); recombinant inbred line (RIL); heterogeneous inbred family (HIF)